Abstract: In order to comprehend the characteristics of antibiotic resistance genes (ARGs) in the matrix of sediment from artificial wetlands and ecological risks, the present study selected the sediments of the Tianjin Lingang constructed wetland as the research object. The sediments were analyzed using high-throughput fluorescence quantitative PCR to examine ARGs. The study then synthesized the main influencing factors and ecological risks. The results showed that a total of 22 ARGs were detected, with higher absolute abundance of sul1, cphA, and aac(6′)-Ib. A total of nine MGEs were detected, with intI-1 exhibiting the highest absolute abundance. Proteobacteria, Firmicutes, and Bacteroidota were the dominant microbial groups in the sediments. The dominant bacteria had some correlation with ARGs and might be potential hosts of ARGs, and some bacteria had multi-drug resistance. Both MGEs and microbial groups were the main influencing factors. The results of the risk assessment indicated that sulfonamides and aminoglycosides were at medium risk; β-lactams and tetracyclines were at medium-high risk at individual sites; while the total ARGs were at low risk; and the ARGs at individual stations had potential hazards and ecological impacts, which should be brought to the attention by the relevant departments for precautionary measures.