光照、温度和氮磷限制对6种典型鱼毒性藻类生长及产毒的影响

曹洁茹; 桓清柳; 吴霓; 江天久

doi:10.13634/j.cnki.mes.2015.03.001

光照、温度和氮磷限制对6种典型鱼毒性藻类生长及产毒的影响

暨南大学赤潮与海洋生物学研究中心;广东省高校水体富营养化与赤潮防治重点实验室, 广东广州 510632

基金项目:

国家自然科学基金(41276091)

国家海洋公益专项(201305003-2)

国家973项目(2010CB428702)

详细信息

作者简介:
曹洁茹(1990-),女,河南许昌人,硕士研究生,研究方向为赤潮毒理学,E-mail:caojieru00@163.com

通讯作者:
江天久,E-mail:tjiangtj@jnu.edu.cn

中图分类号: Q178.53
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出版历程
- 收稿日期: 2014-10-20
- 修回日期: 2015-01-11
- 刊出日期: 2015-05-09

Effects of temperature,light intensity and nutrient condition on the growth and hemolytic activity of six species of typical ichthyotoxic algae

Research Center of Harmful Algae and Marine Biology; Key Laboratory of Eutrophic Water and Harmful Algae Blooms Control and Mitigation, Guangdong High Education Office, Jinan University, Guangzhou 510632, China

摘要

摘要: 为了探究温度、光照、和营养盐对鱼毒性藻类产毒的影响,本文分析了6种我国沿海典型具溶血活性的鱼毒性藻类在不同温度(15℃,25℃,30℃)、光照强度(20 mol/m2/s,60 mol/m2/s,100 mol/m2/s)、氮磷限制(1:1;1:16;1:128)条件下的生长和溶血活性的变化。结果表明,鱼毒性藻类的溶血活性在不同生长期差异明显。海洋卡盾藻(香港株)(Chattonella marina)、卵圆卡盾藻(C.ovata)、米氏凯伦藻(Karenia mikimotoi)、赤潮异弯藻(Heterosigma akashiwo)在对数期溶血活性较高,衰亡期溶血活性较低,而球形棕囊藻(Phaeocystis globosa)与小定鞭藻(Prymnesium parvum)在稳定期溶血活性最高,对数期溶血活性最低。藻类的培养环境和营养条件对鱼毒性藻类溶血毒素的合成有明显的影响,在光照强度、温度和氮磷限制条件下,藻类单个细胞的溶血活性会发生明显变化,6种藻类的溶血活性均随光照强度的升高而增强,在培养温度15~30℃范围内,除球形棕囊藻外,其他5种藻类的单个藻细胞溶血活性随温度的上升而增加,氮磷限制可促进溶血毒素的合成。
- 鱼毒性藻类 /
- 溶血毒素 /
- 环境因子 /
- 氮磷限制
Abstract: Effects of temperature (15,25 and 30℃),light intensity (20,60 and 100 mol/m2/s),nitrogen and phosphorus ratio (1:1; 1:16; 1:128) on hemolytic toxin production of six species of ichthyotoxic algae were studied under laboratory conditions.The results showed that the hemolytic activities of the ichthyotoxic algae in different growth phases were significantly different (p0.05). Chattonella marina,C.ovata,Karenia mikimotoi and Heterosigma akakshiwo exhibited a higher hemolytic activity in exponential phase and a lower hemolytic activity in decline phase.However,Phaeocystis globosa and Prymnesium parvum reached the highest hemolytic activity in stationary phase,and the lowest hemolytic activity of the two algae was detected in exponential phase.The physical environmental factors and nutrition conditions of algae culture also had great effects on the hemolytic activity.The hemolytic activity of single algal cell changed obviously under different conditions of light intensity,temperature,nitrogen and phosphorus limitation.The hemolytic activities of the six algae were positively correlated with light intensities.Except for P.globosa,the hemolytic activities of the other five ichthyotoxic algae showed an increasing trend in temperature range of 15~30℃.Nitrogen and phosphorus limitation promoted the product of hemolytic toxins.
- ichthyotoxic algae /
- hemolytic toxin /
- environmental factor /
- nitrogen and phosphorus limitation

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参考文献(1)

[1]

郭皓,林凤翱,刘永健,等.近年来我国海域多发性赤潮生物种类以及赤潮风险指数分级预警方法[J].海洋环境科学,2014,33(1):94-98.[2]刘洁生,彭喜春,杨维东.营养胁迫下球形棕囊藻(Phaeocystis globosa Scherffel)的生长行为及溶血活性[J].生态学报,2006,26(3):780-785.[5]齐雨藻.中国南海赤潮研究[M].广州:广东经济出版社,2008.[6]彭喜春,刘洁生,杨维东.赤潮藻毒素生物合成研究进展[J].热带亚热带植物学报,2006,14(1):81-86.[8]刘望鹏,黄娟,杨维东,等.营养盐对海洋卡盾藻生长与产毒的影响[J].生态毒理学报,2010,5(3):394-401.[10]江涛,王锐,吴霓,等.海洋卡盾藻(香港株)溶血活性特征研究[J].环境科学,2011,32(10):2920-2925.[11]彭颖慧,刘洁生,李宏业,等.赤潮藻溶血活性测定标准的建立[J].卫生研究,2009,38(6):653-656.[13]YASUMOTO T,UNDERDAL B,AUNE T,et al.Screening for hemolytic and ichthyotoxic components of Chrysochromulina polylepis and Gyrodinium aurolum from Norwegian coastal waters[M].New York:Elsevier Science Publications,1990:436-442.[14]郭瑾,杨维东,刘洁生,等.温度、盐度和光照对球形棕囊藻生长和产毒的影响研究[J].环境科学学报,2007,27(8):1341-1346.[20]BRUYANT F,RYAN C M,NORMANDEAU C,et al.Changes in the haemolytic properties of Heterosigma akashiwo (Raphidophyceae) during phosphate starvation[C]//ASLO Summer Meeting 2005:A Pilgrimage through Global Aquatic Sciences.Santiago de Compostela (abstract).

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