Preparation and identification of monoclonal antibody against Gonyautoxins GTX1-4

HUANG Bai-qiang; ZHANG Zhuo; JIANG Tian-jiu

doi:10.12111/j.mes.20200042

Volume 40 Issue 3

Jun. 2021

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Chinese Journal of MARINE ENVIRONMENTAL SCIENCE > 2021 > 40(3): 457-461, 484. > DOI: 10.12111/j.mes.20200042

HUANG Bai-qiang, ZHANG Zhuo, JIANG Tian-jiu. Preparation and identification of monoclonal antibody against Gonyautoxins GTX1-4[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2021, 40(3): 457-461, 484. DOI: 10.12111/j.mes.20200042

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Preparation and identification of monoclonal antibody against Gonyautoxins GTX1-4

Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Research Center of Harmful Algae and Marine Biology, Jinan University, Guangzhou 510632, China

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Received Date: February 27, 2020
Revised Date: March 28, 2020

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Abstract

The goal of this study was to develop a method for preparation of monoclonal antibody against Gonyautoxins GTX1-4. GTX1-4 artificial antigen was synthesized by formaldehyde method, and GTX1-4 monoclonal antibody was prepared by mouse immunity and monoclonal antibody technology. The results showed that the conjugated ratios of immunizing antigen GTX1-4-BSA and detected antigen GTX1-4-KLH were 10.7∶1 and 22.4∶1, respectively. Three hybridomas cell lines, 1-A4, 5-A11 and 5-E11, which could stably secret GTX1-4 monoclonal antibody were screened. The subtypes of monoclonal antibodies in ascites induced by 5-E11 and 1-A4 were IgG1 and IgG2a, and the titers of them were up to 64000. The subtype of monoclonal antibody in ascites induced by 5-A11 was IgG1, and its titer could be 128000. The sensitivity of monoclonal antibody in ascites induced by 5-A11 was 6.25 μg/mL, and this antibody has a strong specificity. In the study, consequently, GTX1-4 artificial antigen was successfully synthesized, and the hybridomas cell lines which could stably secret GTX1-4 monoclonal antibody was obtained.
- paralytic shellfish poison toxins,
- gonyautoxins,
- complete antigen,
- GTX1-4 monoclonal antibody

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