Citation: | YE Zhi-lin, CAO Jie-ru, WU Ni, JIANG Tian-jiu. Effects of temperature, light intensity and salinity on growth and toxin production of paralytic shellfish poisoning toxins-producing dinoflagellates[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2018, 37(3): 321-325, 348. DOI: 10.12111/j.cnki.mes20180301 |
Experiments were carried out to investigate the effects of light intensity(60, 120 and 200 μmol/(m2·s)), temperature(16, 22 and 28℃) and salinity(25, 30 and 35) on growth and paralytic shellfish poisoning(PSP) toxins production by Alexandrium tamarense HongKong strain(ATHK), A. minimum Taiwan strain(AMSY), A. catenella Nanhai strain(ACSY) and Gymnodinium catenatum Fangchenggang strain(GCFC). The results showed that 22℃ was the optimum growth temperature for all the four strains. Low temperature promoted PSP toxins production in all of the four strains. Except for GCFC, PSP toxins production in the other three strains were reduced with the temperature rising. The growth of ATHK and AMSY were positively correlated with light intensity. Toxin production in AMSY and GCFC were also positively correlated with light intensity. Toxicity of both ATHK and ACSY were also affected by light intensity, with maximum toxicity obtained at the light intensity of 120 μmol/(m2·s). Low light intensity inhibited toxin production in all of the four strains. ATHK, AMSY and GCFC all grew well at the salinity of 30, while ACSY grew well at the salinity of 35. Toxin production in ATHK increased with the increasing salinity. Both higher and lower salinities promoted the toxins production in AMSY and ACSY. A high level of toxin was detected in GCFC at the salinity of 30.
[1] |
ETHERIDGE S M, ROESLER C S.Effects of temperature, irradiance, and salinity on photosynthesis, growth rates, total toxicity, and toxin composition for Alexandrium fundyense isolates from the Gulf of Maine and Bay of Fundy[J].Deep Sea Research Part Ⅱ:Topical Studies in Oceanography, 2005, 52(19/20/21):2491-2500.
|
[2] |
NAVARRO J M, MUÑOZ M G, CONTRERAS A M.Temperature as a factor regulating growth and toxin content in the dinoflagellateAlexandrium catenella[J].Harmful Algae, 2006, 5(6):762-769. doi: 10.1016/j.hal.2006.04.001
|
[3] |
OSHIMA Y. Post-column derivatization HPLC methods for paralytic shellfish poisons[M]//HALLEGRAEFF G M, ANDERSON D M, CEMBELLA A D, et al. Manual on Harmful Marine Microalgae. IOC Manuals and Guides No. 33. France: UNESCO, 1995: 81-94.
|
[4] |
ANDERSON D M, KULIS D M, QI Y Z, et al.Paralytic shellfish poisoning in southern China[J].Toxicon, 1996, 34(5):579-590. doi: 10.1016/0041-0101(95)00158-1
|
[5] |
江天久, 黄伟建, 王朝晖, 等.几种环境因子对塔玛亚历山大藻(大鹏湾株)生长及其藻毒力影响[J].应用与环境生物学报, 2000, 6(2):151-154. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=yyyhjswxb200002011
|
[6] |
韩华, 周春, 于光磊, 等.大窑湾海域虾夷扇贝体内麻痹性贝毒的周年变化[J].海洋环境科学, 2012, 31(3):436-438. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hyhjkx201203027
|
[7] |
江天久, 尹伊伟, 骆育敏, 等.广东大亚湾和大鹏湾麻痹性贝类毒素研究[J].中国环境科学, 2000, 20(4):341-344. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zghjkx200004013
|
[8] |
HWANG D F, LU Y H.Influence of environmental and nutritional factors on growth, toxicity, and toxin profile of dinoflagellate Alexandrium minutum[J].Toxicon, 2000, 38(11):1491-1503. doi: 10.1016/S0041-0101(00)00080-5
|
[9] |
ANDERSON D M, KULIS D M, SULLIVAN J J, et al.Dynamics and physiology of saxitoxin production by the dinoflagellates Alexandrium spp.[J].Marine Biology, 1990, 104(3):511-524. doi: 10.1007/BF01314358
|
[10] |
OGATA T, KOIKE K, NOMURA S, et al. Utilization of organic substances for growth and toxin production by Alexandrian tamarense[M]//YASUMOTO T, OSHIMA Y, FUKUYO Y. Harmful and Toxic Algal Blooms. Paris: Intergovernmental Oceanographic Commission of UNESCO, 1996: 343-346.
|
[11] |
GRIBBLE K E, KEAFER B A, QUILLIAM M A, et al.Distribution and toxicity of Alexandrium ostenfeldii (Dinophyceae) in the Gulf of Maine, USA[J].Deep Sea Research Part Ⅱ:Topical Studies in Oceanography, 2005, 52(19/20/21):2745-2763. https://www.researchgate.net/publication/305904060_Human_impact_in_Mediterranean_coastal_ecosystems_and_climate_change_emerging_toxins
|
[12] |
CIMINIELLO P, DELL'AVERSANO C, FATTORUSSO E, et al.Toxin profile of Alexandrium ostenfeldii (Dinophyceae) from the Northern Adriatic Sea revealed by liquid chromatography-mass spectrometry[J].Toxicon, 2006, 47(5):597-604. doi: 10.1016/j.toxicon.2006.02.003
|
[13] |
TOMAS C R, VAN WAGONER R, TATTERS A O, et al.Alexandrium peruvianum (Balech and Mendiola) Balech and Tangen a new toxic species for coastal North Carolina[J].Harmful Algae, 2012, 17:54-63. doi: 10.1016/j.hal.2012.02.011
|
[14] |
GU H F.Morphology, phylogenetic position, and ecophysiology of Alexandrium ostenfeldii (Dinophyceae) from the Bohai Sea, China[J].Journal of Systematics and Evolution, 2011, 49(6):606-616. doi: 10.1111/jse.2011.49.issue-6
|
[15] |
刘青, 刘冰莉, 王仁锋, 等.光照强度和盐度对塔玛亚历山大藻生长的影响[J].海洋环境科学, 2015, 34(2):199-205. http://hyhjkx.nmemc.org.cn/CN/abstract/abstract44.shtml
|
[16] |
LIM P T, USUP G, LEAW C P, et al.First report of Alexandrium taylori and Alexandrium peruvianum (Dinophyceae) in Malaysia waters[J].Harmful Algae, 2005, 4(2):391-400. doi: 10.1016/j.hal.2004.07.001
|
[17] |
GRZEBYK D, BÉCHEMIN C, WARD C J, et al.Effects of salinity and two coastal waters on the growth and toxin content of the dinoflagellate Alexandrium minutum[J].Journal of Plankton Research, 2003, 25(10):1185-1199. doi: 10.1093/plankt/fbg088
|
[18] |
HAMASAKI K, HORIE M, TOKIMITSU S, et al.Variability in toxicity of the dinoflagellate Alexandrium Tamarense isolated from Hiroshima Bay, Western Japan, as a reflection of changing environmental conditions[J].Journal of Plankton Research, 2001, 23(3):271-278. doi: 10.1093/plankt/23.3.271
|
[19] |
PARKHILL J P, CEMBELLA A D.Effects of salinity, light and inorganic nitrogen on growth and toxigenicity of the marine dinoflagellate Alexandrium tamarense from northeastern Canada[J].Journal of Plankton Research, 1999, 21(5):939-955. doi: 10.1093/plankt/21.5.939
|
[20] |
LAABIR M, COLLOS Y, MASSERET E, et al.Influence of environmental factors on the paralytic shellfish toxin content and profile of Alexandrium catenella (Dinophyceae) isolated from the mediterranean sea[J].Marine Drugs, 2013, 11(5):1583-1601. doi: 10.3390/md11051583
|
[21] |
MIHALI T K, CARMICHAEL W W, NEILAN B A.A putative gene cluster from a Lyngbya wollei bloom that encodes paralytic shellfish toxin biosynthesis[J].PLoS One, 2012, 6(2):e14657. http://downloads.hindawi.com/journals/bmri/2013/463894.xml
|
[22] |
MIHALI T K, KELLMANN R, NEILAN B A.Characterisation of the paralytic shellfish toxin biosynthesis gene clusters inAnabaena circinalis AWQC131C and Aphanizomenon sp.NH-5[J].BMC Biochemistry, 2009, 10:8. doi: 10.1186/1471-2091-10-8
|
[23] |
ANDERSON D M, ALPERMANN T J, CEMBELLA A D, et al.The globally distributed genus Alexandrium:multifaceted roles in marine ecosystems and impacts on human health[J].Harmful Algae, 2012, 14:10-35. doi: 10.1016/j.hal.2011.10.012
|
[1] | ZHANG Xue-wei, HAN Zhen. Prediction and analysis of Argo temperature data by ConvGRU model[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2022, 41(4): 628-635. DOI: 10.12111/j.mes.20210054 |
[2] | YANG Zhi, YE Jin-qing, YANG Qing, GUO Hao. Zooplankton diversity and its relationships with environmental factors in the Liaohe estuary[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2020, 39(1): 25-30. DOI: 10.12111/j.mes20200104 |
[3] | ZHANG Nan-nan, DING Guang-hui, LI Xue-yao, LIU Quan-bin, XUE Huan-huan, CUI Fu-xu, ZHANG Jing. Study on sedimentation of graphene oxide in aqueous matrices with different salinity[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2019, 38(2): 238-243. DOI: 10.12111/j.mes20190211 |
[4] | QIN Guang-xiong, HU Xiao-nong, ZHANG Xiao-ying, HAO Yan-ru, LI Dan, CHEN Kun. Characterization of groundwater salinity spatial variation in the Pearl River estuary[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2018, 37(6): 835-842. DOI: 10.12111/j.mes20180606 |
[5] | YANG Yi-yi, LI Yi, YE Shu-feng. Studies on the ecological threshold of phytoplankton to the environmental gradient of salinity in the Yueqing bay, Zhejiang province, China[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2018, 37(4): 499-504. DOI: 10.12111/j.cnki.mes20180405 |
[6] | LIU Zi-wen, WEI Xue-xin, XU Yun-kai, GE Zhen-ming, WANG Dong-qi. Effects of salinity on methane emission at Yangtze Estuary Phragmites australis wetland[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2018, 37(3): 356-361, 388. DOI: 10.12111/j.cnki.mes20180307 |
[7] | WANG Zhong-yuan, ZHANG Hong-kang, YU Han-sheng, LI Xiao-min, CAI Jian-wen, CAI Si-si. Determination of dissolved trace Zn in seawater with different salinity by a hig speed separation and preconcentration method in a knotted reactor coupled with flame atomic absorption spectrometry[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2016, 35(4): 618-622. DOI: 10.13634/j.cnki.mes20160422 |
[8] | WANG Chuan-jun, WU Ying-chao, WANG Yu-guang, FU Yuan-bin, LI Qing, YU Ji, CAI Yue-yin, GONG Wei. Distribution of soil salinization and analysis of salt characteristics in the coastal areas of Liaodong Bay[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2015, 34(6): 937-941. DOI: 10.13634/j.cnki.mes.2015.06.022 |
[9] | ZHANG Yi-hui, WEI Qing-fei, WANG Yu-guang, WANG Chuan-jun. Application of electric conductivity method in seawater intrusion and soil salinization[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2015, 34(4): 593-595. DOI: 10.13634/j.cnki.mes.2015.04.021 |
[10] | LIU Qing, LIU Bing-li, WANG Ren-feng, ZHANG Mo, CUI Yan-chao, XU Dong-hai. Effect of salinity and illumination on the growth of Alexandrium tamarense[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2015, 34(2): 199-205. DOI: 10.13634/j.cnki.mes.2015.02.008 |